For Prince Harry, who was just 12 when his mother was killed in a car crash in Paris, the loss will be keenly felt on the day he marries Meghan Markle.
All of Diana's siblings will attend - Earl Spencer, Lady Sarah McCorquodale and Lady Jane Fellowes - and Lady Jane will give a reading.
The engagement ring Prince Harry designed for his fiancee contains two diamonds from Diana's personal collection.
He said he wanted the princess to be part of his and Ms Markle's "crazy journey together".
"It is days like today when I really miss having her around, and miss being able to share the happy news," the prince said when the pair announced their engagement in November.
Ms Markle echoed his thoughts saying: "She's with us."
Asked what his mother would have thought of the US actor, the prince said "they'd be thick as thieves, without question".
Diana was only 36 when she was killed in a car crash in Paris in 1997 while being pursued by the paparazzi and driven by a drunk chauffeur.
The image of her young sons walking behind their mother's coffin through the streets of London on the day of her funeral has become etched in the public's memory.
In 2017 Prince Harry said he had come close to a complete breakdown on numerous occasions.
He revealed he sought counselling after two years of "total chaos", having spent nearly 20 years "not thinking" about the death of his mother.
He set up his charity Sentebale in her memory in 2006.